Service detail- Precedo ltd.- leading CRO company

STAT6/SPR/PDX-PDO/SELEX/Immune-Organoid Co-culture

Focus on the core technologies of cell engineering related to national strategic biosecurity and industrial security, and carry out scientific and technological research and industrialization around the bottleneck of biomedicine development - the "cell" problem

STAT6 Detection Service List

Service items	Testing method
Affinity test	Detect the affinity of binary and ternary complexes through SPR (Surface Plasmon Resonance) technology
Detection of the STAT6/CRBN ternary complex formation	Using the Alphalisa technology, the binding of the drug to STAT6/CRBN to form a ternary complex was detected.
Protein Degradation Test	Detect the degradation efficiency of STAT6 by the drug in A549-STAT6-HiBiT cells
Protein Degradation Test	Using the WB method to detect the degradation level of STAT6 by the drug in cells such as MV4-11 and Jurkat
Inhibition of signaling pathways	Detection of the inhibitory effect of the drug on the phosphorylation of STAT6 induced by IL-4/IL-13
Inhibition of signaling pathways	Detect the inhibition of drugs on the expression of CD23 induced by IL-13 and the release of CCL17
Selective testing	Evaluation of the selectivity of the drug for STAT6 (Total STAT, pSTAT, reporter gene)
Pharmacokinetics	Detect the pharmacokinetic parameters of the drug in the mice' bodies
Single-dose pharmacodynamics study	Detect the degradation of STAT6 in the whole blood and spleen of mice after a single administration.
Intracellular efficacy	To detect the efficacy of the drug in the MC903-induced hIL-4/hIL-4R mouse model of Alzheimer's disease (AD)
Intracellular efficacy	Detect the efficacy of the drug in the asthma model induced by HDM